Vancouver's northern hemisphere campaign is over before it started and he will now return to Australia to begin his stud career.
Trained by Gai Waterhouse to win last year's Golden Slipper, Vancouver was bought by Coolmore and sent to Aidan O'Brien in Ireland with ambitions to add a northern hemisphere Group One win to his record.
But Coolmore has decided to scrap plans to run him in the Diamond Jubilee at Royal Ascot after he missed a recent run because of an unsatisfactory blood picture.
"His bloods failed to improve as quickly as we might have hoped so he missed more work last week and this week," O'Brien told the Racing Post.
"Unfortunately we've now run out of time to get him ready for the Diamond Jubilee and the July Cup.
"It's very disappointing as he looked very special on what we'd seen of him at home and we were looking forward to running him."
Coolmore Australia boss Tom Magnier said Vancouver would now head home.
"It's a major disappointment that Vancouver won't be running at Royal Ascot but he is proving very popular with breeders so we're keen to get him here as soon as possible," he said.
"He'll enter quarantine in Newmarket next week and will be on the farm here in Coolmore Australia by mid-July."