Frankel has had his first mare scanned in foal at Banstead Manor Stud in Newmarket.
The mare is the former David Wachman-trained Chrysanthemum, a dual Group Three winner and third in the Group One Pretty Polly Stakes at the Curragh in 2011.
Frankel, who retired at the end of last season undefeated after 14 races and became the highest-rated horse in the world, has an outstanding book of mares in his first season as a stallion.
Bated Breath, also owned by Khalid Abdullah, has had his first mare tested in foal - Pretty Primo, a winning half-sister to Group One winner Wootton Bassett.
"Both horses are proving to be very professional and it is always good to get their first mare tested in foal," stud general manager Philip Mitchell said on the Juddmonte website.
Among the mares served by Frankel are Prix de l'Arc de Triomphe winner Danedream and Japanese champion Vodka.
He is standing his first season at STG125,000 ($A185,763).