Telescope has a sore left shin and will not run in the Derby at Epsom on June 1, trainer Sir Michael Stoute has announced.
The Galileo colt had a racecourse gallop at Lingfield on Wednesday in which he was described as only "workmanlike" by the Newmarket handler.
He has since developed a sore shin with Stoute of the opinion the premier Classic will now come too soon.
"He has a bit of soreness on a left shin and I don't think the horse will be going to Epsom," Stoute said.
"He's not giving us the right signs and won't be running."
Telescope was the leading British hope for the Derby with Irish colt Dawn Approach now at 4-5 to win.