Favourite Atlantic Jewel has been scratched from the Cox Plate with a tendon injury.
The mare worked well at Flemington on Tuesday morning but was found to have swelling in a flexor tendon in her off side foreleg when she cooled down.
Owners Coolmore will make a decision on the mare's future in the next few weeks.
Atlantic Jewel has won 10 of her 11 races with It's A Dundeel, the new favourite for Saturday's Cox Plate, beating her in last month's Underwood Stakes.