Melbourne Cup-winning trainers Mikel Delzangles and Gai Waterhouse will front authorities on Friday to answer charges relating to raceday treatment given to Dunaden and Tres Blue.

French trainer Delzangles will be asked to explain why 2011 Cup winner Dunaden, 11th in Tuesday's race, received an ulcer treatment on race morning.

Waterhouse, trainer of this year's Cup winner Fiorente, has been called over an antibiotic cream applied to the hooves of her other runner Tres Blue who finished near the tail of the field.

Both trainers admitted the treatments had been given, in both cases by stablehands, and both horses were allowed to run in the Cup with stewards satisfied neither substance contained illegal ingredients.

Mandatory minimum penalties of six months disqualification for treating horses on race day were introduced earlier this year with stewards given discretion to allow for any special circumstances.

Victoria's chief steward Terry Bailey said had the Waterhouse stable asked permission to apply the paste, it would have been granted.

But in the case of Dunaden, stewards would have declined a request from Delzangles.

"The administration of the paste to Dunaden is a daily routine used as ulcer treatment but if they had made application it would have been denied," chief steward Terry Bailey said.

Bailey also said there could be no comparison between the new cases and that of Adelaide trainer Paul Beshara who was banned for six months after being found guilty of giving Happy Trails an injection of an unknown substance on the morning he was due to race in September.

"In the Beshara case, it was clear the horse had received an injection," Bailey said.

"The evidence was overwhelming.

"The trainer denied it therefore we weren't able to establish what it had been injected with.

"That's a whole different scenario."

Stewards formally issued the charges on Wednesday with the trainers to appear before the Racing Appeals and Disciplinary Board on Friday afternoon.