Exceptional miler Kingman has been retired and will stand at Banstead Manor Stud in 2015 after failing to recover from a throat infection.
The John Gosden-trained colt was being trained for the Queen Elizabeth II Stakes at Ascot on Champions Day.
Connections still hoped the three-year-old would make the showpiece race, but time has now been called on a decorated career.
Owner Prince Khalid Abdulla's racing manager Teddy Grimthorpe said in a statement the throat infection still required treatment.
"The throat infection will still need on going treatment which will rule out the Queen Elizabeth II Stakes and also the Breeders Cup," Grimthorpe said.
"The stud fee of the horse that has electrified European racing in 2014 will be announced by the stud when appropriate."
Kingman won seven of his eight races, including Group One triumphs in the Irish 2000 Guineas, the Sussex Stakes at Goodwood, the Prix Jacques le Marois at Deauville and the St James's Palace Stakes at Royal Ascot.
He will stand alongside the incomparable Frankel at Banstead Manor.