Red Tracer will have to overcome barrier 14 of 17 to defend her title in the Tattersall's Tiara at Eagle Farm.
The fillies and mares race over 1400 metres on Saturday is the final Group One race of the Australian season.
Trainer Chris Waller will have three runners with Catkins (barrier one) and She's Clean (barrier 12) joining Red Tracer.
Gai Waterhouse's pair Driefontein and Cosmic Endeavour have drawn well in gates three and five respectively.